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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which results in chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was observed to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial function in advertising cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two more splice variants had been documented but specific nucleotide sequence information just isn't nevertheless available.
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Blocking peptides are peptides that bind specially towards the concentrate on antibody and block antibody binding. These peptide ordinarily contains the epitope recognized because of the antibody.
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This guideline outlines even further possible optimization of cellular and immunoassay parameters to be sure the very best outcomes are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to be sure the very best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope over the target protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is precise.
Focus on Data Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and features to be a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates on read more the G1-S section boundary which is degraded as cells progress by S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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